Taxonomic complexity analysis of the Chlorella clade (Chlorellaceae)

In this study, 253 of 18S rDNA sequences, 155 ITS2 63 rbcL and 23 tufA sequences were collected on GenBank to evaluate analyse the taxonomic complexity Chlorella clade. C. vulgarisand sorokiniana had most DNA barcoding. Analysis phylogenetic tree results showed that strains these two species divided into 2 3 small groups with genetic differences. addition, different belonging same clade in trees observed. For example, Actinastrum hantzschii, chlorelloides, singularis, sorokiniana, thermophila, volutis, vulgaris, Micractinium pusillum, M. reisseri belonged group tree. This taxonomy came not only from cryptic species, but also relationships remaining species. Therefore, identifying determining relationship have been still challenging, because database as well incomplete system. Clarification is needed improve identification new isolates